Transfers Wenger shuts down further Sanchez to City talk Joe Wright 04:18 1/1/18 FacebookTwitterRedditcopy Comments() Getty Images Transfers Manchester City Arsène Wenger A. Sánchez West Bromwich Albion v Arsenal Arsenal West Bromwich Albion Premier League Crystal Palace v Manchester City With speculation continuing to mount about the Chilean’s future, the Arsenal boss is growing short on patience Arsene Wenger angrily shut down questions about Alexis Sanchez’s Arsenal future amid persistent rumours of renewed interest from Manchester City.City failed in their pursuit of the Chile star during the previous transfer window but there is strong speculation they will attempt to strike a deal next month, with Sanchez’s contract expiring at the end of the season.Gabriel Jesus picked up an injury in the 0-0 draw with Crystal Palace on Sunday that manager Pep Guardiola says could keep him out for up to two months, encouraging talk of an imminent City bid for Sanchez Article continues below Editors’ Picks Lyon treble & England heartbreak: The full story behind Lucy Bronze’s dramatic 2019 Liverpool v Man City is now the league’s biggest rivalry and the bitterness is growing Megan Rapinoe: Born & brilliant in the U.S.A. A Liverpool legend in the making: Behind Virgil van Dijk’s remarkable rise to world’s best player Guardiola himself stated he expects the 29-year-old to stay at the Emirates Stadium, though, and Wenger was in no mood to discuss the issue further after his side were held to a 1-1 draw at West Brom courtesy of Jay Rodriguez’s controversial late penalty.Asked if Sanchez had played his last game for Arsenal at The Hawthorns, Wenger told a news conference: “We play on Wednesday [against Chelsea].”I have spoken many times about that. I’m not ready to talk about that some more.”I have given you my answer many times. I don’t see why I should come back to a subject we have talked about 155 times.”The year ends with @ManCity’s gap at 14 points pic.twitter.com/ahOjW4gpfr— Premier League (@premierleague) December 31, 2017Wenger also hit back at club legend Thierry Henry after his suggestion that Arsenal players’ mixed celebrations during the 3-1 win at Crystal Palace this week pointed to a dressing-room rift caused by Sanchez’s uncertain future.”It was never a problem inside [the club],” he said. “I don’t listen to what he says and other people says.”We focus on our own problems, that’s the only thing.”There are people who are paid to talk. It’s difficult sometimes when you are paid to talk, to talk, to talk and only say things that are true and intelligent.”Asked if Arsenal will look to make signings in the transfer window, Wenger added: “Tomorrow [Monday], I will try to bring myself in first and, after that, we will see.”
WILMINGTON, MA — The Wilmington Community Fund’s 2018 Holiday Ornament has gone is now on sale. The holiday ornament is available at Designs By Don (380 Main Street) and at the Recreation Department Office at Town Hall. The $10 ornament will also be sold at the upcoming WOW’s Festival of Trees at Villanova Hall on December 1-2 and the Town’s Annual Tree Lighting on the Town Common on December 1.Last year’s ornament is available for $5, while prior years’ ornaments are available for just $1.All proceeds will benefit the Wilmington Community Fund. The Fund has three major goals: (1) provide direct relief to Wilmington families, individuals, the elderly and children; (2) provide basic needs — heat, light, utilities, emergency assistance and medical relief; and (3) provide food products on a bimonthly basis to those in need.2018 Wilmington Holiday Ornament(NOTE: The above information is from the Wilmington Community Fund.)Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email firstname.lastname@example.org.Share this:TwitterFacebookLike this:Like Loading… RelatedPHOTO: First Look At 2017 Wilmington Holiday OrnamentIn “Photo of the Day”PHOTO: First Look At 2016 Wilmington Holiday OrnamentIn “Community”PHOTO OF THE DAY: First Look At 2015 Wilmington Holiday OrnamentIn “Photo of the Day”
Toward a pill to enable celiac patients to eat foods containing gluten © 2013 Phys.org Explore further More information: Sustained gastrointestinal activity of dendronized polymer–enzyme conjugates, Nature Chemistry (2013) doi:10.1038/nchem.1675AbstractMethods to stabilize and retain enzyme activity in the gastrointestinal tract are investigated rarely because of the difficulty of protecting proteins from an environment that has evolved to promote their digestion. Preventing the degradation of enzymes under these conditions, however, is critical for the development of new protein-based oral therapies. Here we show that covalent conjugation to polymers can stabilize orally administered therapeutic enzymes at different locations in the gastrointestinal tract. Architecturally and functionally diverse polymers are used to protect enzymes sterically from inactivation and to promote interactions with mucin on the stomach wall. Using this approach the in vivo activity of enzymes can be sustained for several hours in the stomach and/or in the small intestine. These findings provide new insight and a firm basis for the development of new therapeutic and imaging strategies based on orally administered proteins using a simple and accessible technology. Journal information: Nature Chemistry Citation: Polymers could help enzymes treat diseases (2013, June 10) retrieved 18 August 2019 from https://phys.org/news/2013-06-polymers-enzymes-diseases.html Enzymes lose their structure and break up into their component parts easily. To correct this problem, biochemists have been attaching polymers to enzymes for decades. This makes the enzymes stable enough for use in pharmaceuticals. Doctors tend to administer these modified enzymes by injection or other non-oral routes, so they do not have to pass through the GI tract. Some oral medications contain enzymes; these have coatings to prevent stomach acid from attacking them. However, it is hard to predict how well the coatings will work in individual patients, so such medicines are not very reliable.Recently, scientists modified an enzyme that breaks down phenylalanine, the amino acid that causes a problem for people with phenylketonuria (PKU), by combining it with the polymer polyethylene glycol (PEG). When given orally, this polymer-enzyme conjugate helped reduce phenylalanine levels in mice with PKU. Leroux’s team, which had been studying celiac disease, tried to see if it could achieve similar results by attaching polymers to enzymes that break down gluten, the protein that triggers the disease. An enzyme that digests gluten before it reaches the small intestine would benefit celiac sufferers greatly. Enzymes known as proline-specific endopeptidases (PEPs) break down gluten. However, they are denaturized and deactivated in the stomach. The researchers tried to stabilize different types of PEPs by attaching different polymers to them. They labeled the modified enzymes with a fluorescent substance and fed them to rats. Enzymes attached to dendronized polymers, which have a linear main chain and dendron-type side chain, remained active in the rats’ stomachs for more than three hours. The researchers think that the dendronized polymers may provide a protective effect by adhering to the mucus in the stomach lining. They are now performing further studies on mutated enzymes to gain a better understanding of how the polymers prevent enzymes from degrading. Schematic depiction of the behaviour of MX–polymer conjugates in the GI tract. After oral administration, MX–PG1 interacts with the negatively charged gastric mucin layer, which results in strong mucoadhesion. MX–PDL, MX–PAA and MX–mPEG do not (or only weakly) interact with the mucin layer. Credit: (c) Nature Chemistry (2013) doi:10.1038/nchem.1675 (Phys.org) —Conditions such as celiac disease, phenylketonuria, lactose intolerance and exocrine pancreatic disease involve abnormal enzyme activity. Enzymes administered orally could help sufferers. However, because enzymes, like all proteins, break down in the stomach and small intestine, they cannot usually survive in the gastrointestinal (GI) tract long enough to be effective. In a study published in Nature Chemistry, Jean-Christophe Leroux and his colleagues at the Swiss Federal Institute of Technology report they have found polymers that, when attached to enzymes, will prevent the enzymes from degrading in the GI tract. The research paves the way for new medical treatments. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.